Published date: Mar 27 2024

Journal Title: KnE Life Sciences

Issue title: International Conference On Mathematics And Science Education (ICMScE 2022): Life Sciences

Pages: 85–96

DOI: 10.18502/kls.v8i1.15401

Dalia Sukmawatidalia-Sukmawati@unj.ac.idBiology Department, Laboratory of Microbiology, 9th Floor Hasyim Ashari Building, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Jakarta, Indonesia

Alika FirhandiniBiology Department, Laboratory of Microbiology, 9th Floor Hasyim Ashari Building, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Jakarta, Indonesia

Umi KhumaiyaBiology Department, Laboratory of Microbiology, 9th Floor Hasyim Ashari Building, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Jakarta, Indonesia

Dwi Ayu KomsiatunBiology Department, Laboratory of Microbiology, 9th Floor Hasyim Ashari Building, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Jakarta, Indonesia

Syifa Aulia GunadiBiology Department, Laboratory of Microbiology, 9th Floor Hasyim Ashari Building, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Jakarta, Indonesia

Atin SupiyaniBiology Department, Laboratory of Microbiology, 9th Floor Hasyim Ashari Building, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Jakarta, Indonesia

Dwi Ningsih SusilowatiBalai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian, Indonesia Jl. Tentara Pelajar No. 3A, Bogor 16111, Jawa Barat, Indonesia

Shabrina Nida Al HusnaDepartment of Microbiology, School of Life Sciences and Technology, Institut Teknologi Bandung, Bandung, Jawa Barat, Indonesia

Hesham El EnshasyInstitute of Bioproduct Development, Universiti Teknologi Malaysia (UTM), Skudai, Johor Bahru, Malaysia

Daniel Joe DailinInstitute of Bioproduct Development, Universiti Teknologi Malaysia (UTM), Skudai, Johor Bahru, Malaysia

Catur SriherwantoCentre for Applied Microbiology, National Research and Innovation Agency (BRIN), Science and Technology Park, Banten, Indonesia

The presence of pathogenic molds in post-harvested cocoa beans poses a significant problem to most Indonesian farmers. Pathogenic molds secrete phytopathogenic toxins which cause rotting, seriously damaging the cocoa fruits. The common pathogenic molds found in damaged cocoa beans are Phytophthora palmivora and Phytophthora megakarya. These molds are so far controlled by using chemical pesticides, whose long-term use is potentially harmful to human health and the environment. Thus, a healthier and safer alternative is needed, especially using biological agents such as yeasts. Some yeasts produce cellulose-degrading enzymes that can possibly break down the fungal cell wall which is composed mostly of the polysaccharide’s chitin and glucan. In this study, yeasts were isolated during the cocoa fermentation process, followed by screening for cellulolytic activity using direct planting and paste method, and subsequent antagonistic testing by double culture technique. Out of 128 yeast isolates, 77 possessed cellulase activity, with 6 of them having the highest activity index. Antagonistic activity test of these 6 isolates (C1.0.4, C1.1.3, C2.3.10, C2.3.14, C3.5.11, and C3.3.1) against the pathogenic molds resulted in isolate C3.3.1 showing the highest inhibition percentage from the 2

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