KnE Life Sciences

ISSN: 2413-0877

The latest conference proceedings on life sciences, medicine and pharmacology.

Synthesizing Cylic Peptides with Antioxidant Properties using Solid Phase Peptide Synthesis as an Alternative to Natural Product Isolation from Pork

Published date: Dec 27 2022

Journal Title: KnE Life Sciences

Issue title: Science and Technology Research Symposium (SIRES)

Pages: 147–160

DOI: 10.18502/kls.v7i5.12521

Authors:

N. KurniatyEmail: netykurniaty@yahoo.com
Affiliation: 1 Department of Pharmacy, Faculty of Science, Universitas Islam Bandung, Bandung, Indonesia
Biography:

U. RosyidahEmail: N/A
Affiliation: 1 Department of Pharmacy, Faculty of Science, Universitas Islam Bandung, Bandung, Indonesia
Biography:

A. SuparmanEmail: N/A
Affiliation: Department of Pharmacy, Faculty of Science, Universitas Islam Bandung, Bandung, Indonesia
Biography:

R. YuliantiEmail: N/A
Affiliation: Department of Pharmacy, Faculty of Science, Universitas Islam Bandung, Bandung, Indonesia
Biography:

S. SopiahEmail: N/A
Affiliation: Department of Pharmacy, Faculty of Science, Universitas Islam Bandung, Bandung, Indonesia
Biography:

W. M. BaharudinEmail: N/A
Affiliation: Department of Pharmacy, Faculty of Science, Universitas Islam Bandung, Bandung, Indonesia
Biography:

N. Kurniaty - netykurniaty@yahoo.com

U. Rosyidah

A. Suparman

R. Yulianti

S. Sopiah

W. M. Baharudin

Abstract:

Tetrapeptides Ser-Leu-Tyr-Ala and Tyr-Leu-Tyr-Ala are two derivates that have been successfully isolated from the pork and synthesized using Solid Phase Peptide Synthesis (SPPS) method. Tetrapeptide Gly-Ala-Trp-Ala has also been successfully isolated from the Sardinella aurita fish found in Atlantic Ocean using SPPS. This study aims to synthesize tetrapeptide Ser-Leu-Tyr-Ala, Tyr-Leu-Tyr-Ala and Gly-Ala-Trp-Leu using the SPPS method, and to find the antioxidant properties of the synthesized tetrapeptides using DPPH test. The three tetrapeptides have been synthesized using 2-chlorotritilchloride resin as solid phase, Fmoc group protection, and coupling reagent HBTU/HOBt. HR-TOF-MS with m/z was [M+H]+ 453,23 and [2M+H]+ 905,49 for Ser- Leu-Tyr-Ala, [M+H]+114,61 for Tyr-Leu-Tyr-Ala and [M+H]+ 446,23 for Gly-Ala-Trp-Leu. The antioxidant properties of Ser-Leu-Tyr- Ala had an IC50 value of 11130,04 mg/ml, while the antioxidant properties of Tyr-Leu-Tyr-Ala had an IC50 value of 4319,522 mg/ml.

Keywords: solid phase peptide synthesis, antioxidant

References:

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