Published date: Dec 03 2017

Journal Title: KnE Life Sciences

Issue title: The Veterinary Medicine International Conference (VMIC)

Pages: 734-741

DOI: 10.18502/kls.v3i6.1204

Yugenthri A/P Chandranyugen.chan10@gmail.comStudent of Veterinary Medicine Faculty Surabaya, 60115

Herry Agoes HermadiVeterinary Reproduction Department of Veterinary Medicine Faculty, Surabaya, 60115

Eka Pramyrtha HestianahVeterinary Patholohy Department of Veterinary Medicine Faculty, Surabaya, 60115

This research objective was to increase the rate of pregnancy in Bali cattle using eCG and PGF2 14α">  at Buleleng, Bali. The experimental animal used in this study comprised of 18 female Bali cattles, with normal estrus cycle and 18 months old of age. The Bali cattles are all healthy with a body score of at least 2. The Control Group was injected with PGF2α 25 mg intramuscular twice on day 0 and day 11. Treatment Group 1 was injected with PGF2α 25 mg and eCG dosage of 400 IU intramuscular. Treatment Group 2 was injected with PGF2α 25 mg and eCG dosage of 600 IU intramuscular. On day 14, the female Bali cattle both Treatment Group 1 and 2 respectively showed signs of estrus. Few hours’ later artificial insemination was performed on the same day. On Day 30, all 18 female Bali cattle were checked for pregnancy using Ultrasonography (USG). The female Bali cattle were assumed to be pregnant because there were signs of enlargement in cornua uteri unilaterally.  Therefore, this indicates that there was no significant difference between Treatment Group 1 and Treatment Group 2 in pregnancy rate. The results from Control Group showed 4 pregnant and 2 not-pregnant; Treatment Group 1 showed 6 pregnant; Treatment Group 2 showed 6 pregnant. Based on the results, Control Group showed 88% of the Bali cattle were pregnant. Meanwhile, Treatment Group 1 and Treatment Group 2 showed 100% pregnant from the total 18 Bali cattle. Therefore, this research showed a positive feedback consistent with the objective.

[1] Lunenfeld, B. 2004. Historical Perspectives in Gonadotrophin Therapy. Hum. Reprod. Update, 10:453-67.

[2] Gordon, I.R. 2004. Reproductive Technologies in Farm Animals. CABI ISBN 978-05199-862-6.

[3] Adams, H., Richard. 2001. Veterinary Pharmacology and Therapeutics. Blackwell Publishing. ISBN 978-0-8138-1743-9.

[4] Hafez, E.S.E. 2000. Reproduction in Farm Animal 7th ed. Philadelphia. Baltimore. New York London.

[5] Ratnawati, D., D.M. Dikman, and J. Efendy. 2011. Pemanfaatan PMSG Lokal sebagai Alternatif Hormon Superovulasi. Seminar Nasional Teknologi Peternakan dan Veteriner. 32-37.

[6] Duggavathi, R., P.M. Bartlewski, E. Agg, S. Flint, D.M.W. Barret and N.C. Rawlings. 2005. The Effect the Manipulation of Follicle Stimulating Hormone (FSH) Peak Characteristics on Follicular Wave Dynamics in sheep: Does on Ovarian Independent Endogenous Rhythm in FSH Secretion Exist. Boil. Reprod. 72:1466-1474.

[7] de Mestre, A.M., D. Hanlon, A.P Adams, E. Runcan, J.C. Leadbeater, H.N. Erb, C.C. Costa, D. Miller, W.R. Allen, and D.F. Antczak. 2011. Functions of Ectopically Transplanted Invasive Horse Trophoblast. Reproduction, 141:849-856.

[8] Murphy, B.D. 2012. Equine Chorionic Gonadotropin: An Enigmatic but Essential Tool. Journal Animal Reproduction Canada 9: (3). 223 - 230.

[9] Ginther, O.J., J.P. Kastelic and L. Knopt. 1989. Intraovarian Relationships among Dominant and Subordinat Follicles and the Corpus Luteum in Heifers. Theriogenology 32:787-795.

[10] Mahaputra, L., dan L Mustafa. 2002. Kinerja Serum Sapi Birahi dan Kuda Birahi Sebagai Suplemen Media Maturasi Oosit Pada Fertilisasi In Vitro Sapi Madura. Jurnal Biosains Pasca Sarjana Vol.4 no.3. September. 113-117.

[11] Hermadi, H.A., dan Mahaputra, L. 2012. Produksi Equine Chorionic Gonadotropin (cCG) Frozen Dry dari Serum Kuda Bunting Lokal untuk Peningkatan Kebuntingan Sapi Madura. Laporan Penelitian Unggulan Perguruan Tinggi Tahun Anggaran 2012. Universitas Airlangga. Surabaya.

[12] Arthur, G.H. 1993. Veterinary Reproduction and Obstetrics The English Language Book Society and Balliere. Tindal London.