Published date: Dec 03 2017

Journal Title: KnE Life Sciences

Issue title: The Veterinary Medicine International Conference (VMIC)

Pages: 702-711

DOI: 10.18502/kls.v3i6.1200

Dwi Gayatri Nurcahyawatiduwigayatri@gmail.comStudent of Veterinary Medicine Faculty Surabaya, 60115

Hani PlumeriastutiVeterinary Pathology Department of Veterinary Medicine Faculty, Surabaya, 60115

Lilik MaslachahVeterinary Basic Medicine Department of Veterinary Medicine Faculty, Surabaya, 60115

The purpose of this study was to know the effect of Dayak onion tuber extract (Eleutherine palmifolia) given by per oral in lowering levels of histophatology damage kidney of albino male rat (Rattus norvegicus) strain Wistar which was induced by alloxan. Animals which were used in this research were 24 white male rats (Rattus norvegicus) strain Wistar, and then divided into 6 groups. The negative control group K (-) were given with aquabidest and CMC-Na 1% during the therapy period, the positive control group K (+) were given with alloxan 110 mg / kgbw, the group of drug control K (O) were given with alloxan and oral therapy  with metformin 9 mg / 200g bw / day, the treatment group 1 (P1) were given with alloxan and per oral therapy with extract of  Dayak onion tuber 100 mg / kgbw, the treatment group 2 (P2) were given with alloxan and per oral therapy with extract of Dayak onion tuber 200 mg / kgbw and the treatment group 3 (P3) were given with alloxan and per oral therapy with extract of Dayak onion tuber 400 mg / kgbw. The therapy was given for 14 days, then the animals were sacrificed with ketamine and then its kidney was taken for examination of hisphatology in kidney. Observations based on their depiction of renal histopathology tubular degeneration and necrosis, glomerular necrosis, intestitial infiltration and glomerular sclerosis. Data obtained from the scoring of histopathological appearence albino rat kidneys were analyzed by test Kruskal-Wallis and if there is a real difference followed by Mann-Whitney test using SPSS 20.0 for windows. The results showed that the extract of Dayak onion tuber 400 mg/ kgbw  can reduce the degree of kidney damage in albino male rat exposed to alloxan significantly.

[1] ADA (American Diabetes Association). 2013. Standar of Medical Care in Diabetes. 33 (suppl.1): S11.

[2] Lukas, A., Ari, F., Adi, P. 2015Effect of Cow Milk Powder Administration on Hepatic MDA Levels of White Male Rats (RattusNovergicusWistar Strain) Model of Type 2 Diabetes Mellitus.


[3] Rahimi, Z., Hasnvand, A., Fellehgary, V. 2012. Interaction of MTHFR 1298C with ACE D Allele Augments The Risk of Diabetic Nephropathy in Western Iran. DNA Cell Biol. 31 (4):553-9

[4] Hendromartono. 2009. Nefropati Diabetik dalamBuku Ajar IlmuPenyakit Dalam. Edisi IV. Jakarta : Interna Publishing.

[5] Esteghamati, A., Eskandari, D., Mirmiranpour, H., Noshad, S., Mousavizadeh, M., Hedayati, M. 2013. Effects of Metformin on Markers Of Oxidative Stress And Antioxidant Reserve In Patients With Newly Diagnosed Type 2 Diabetes. 32:179-85.

[6] Kasiviswanath, R., A. Rames and K.E. Kumar. 2005. Hipoglycemia and Antihyperglicemia Effect of Gmelina asiatatica Linn. In Normal and Aloxan Induced Diabetic Rats. Biol. Pharm. Bull.28(4) : 729-732.

[7] Galingging, R. Y. (2009). Bawang Dayak (Eleutherine palmifolia) Sebagai Tanaman Obat Multifungsi. Warta Penelitian dan Pengembangan Vol 15, no. 3, Halaman 2-4.

[8] Febrinda, A.E. 2014. Potensi Antioksidan dan Antidiabetik Ekstrak Air dan Etanol Umbi Bawang Dayak (Eleutherine Palmifolia) secara In Vitro dan In Vivo [Tesis]. Fakultas Teknlogi Pertanian Institut Pertanian Bogor.

[9] Klopfleisch, R. 2013. A Short Textbook of Veterinary Oncology.

[10] Kusriningrum. 2008. Perancangan Percobaan. Airlangga University Press. Surabaya. 15 – 97.

[11] Das, J., Vasan, V., Sil, P. C. 2012. Taurine Exerts Hypoglicemic Effect In Alloxan-Induced Diabetic Rats, Improves Insulin-Mediated Glucose Transport Signaling Pathway In Heart And Ameliorates Cardiac Oxidative Stress And Apoptosis. 296-308

[12] Kabel, A. M. 2014. Free Radical and Antioxidants: Role of Enzymes and Nutrition. World Journal of Nutrition and Healt. Vol 2. No. 3, 35-38.

[13] Shargel, L., Wu-Pong, S., Yu. 2005. Applied Biopharmaceutics and Pharmacokinetics. Singapore.

[14] Handani, R. A., Salim, M. N., Harris, A., Budiman, H., Zainuddin. 2015. The Effect of Long Beans (Vigna unguiculate) on Kidney Microscopic Structure of Alloxan Induced Mice (Mus musculus). Jurnal Medika Veterinaria. Vol. 9 No. 1.
[15] Grace-Lynn, C., Darah, I., Chen, Y., Latha, L. Y., Jothy, S. L., Sasidharan, S. 2012. In Vitro Antioxidant Activity Potential Of LAntadene A, A Petacyclic Triterpenoid Of Lantana Plants. Molecules 17: 11185-11198.

[16] Chaturvedi, N. 2007. The Burden Of Diabetes And Its Complications: Trends And Implications For Intervention. Diabetes Res Clin Pract, 76(3):S3-S12.

[17] Lukacinova, A., Mojzis, J., Benacka, J., Keller, Maguth, Kurila, P., Vasko, L., Racz, O., Nistiar, F. 2008. Preventive Effects of Flavonoids on Alloxan-Induced Diabetes Mellitus in Rats. 77:175-182.

[18] Carrara V. S., A. A. Amato., F. A. R. Neves. 2009. Effects of a Methanolic Fraction of Soybean Seeds on the Transcriptional Activity of Peroxisome Proliferator-Activated Reseptors. Journal of Medical and Biological Research. Brazil. 546.

[19] Zachari, J. F. and Mc. Gavin M. D. 2012. Pathologic Basis of Veterinary Disease. Fifth Edition. Elsevier Inc. St. Louis. Missouri.

[20] Ahmadvand, H., Tavafi, M., Khosrowbeygi, A. 2012. Amelioration of Altered Antioxidant Enzymes Activity and Glomerulosclerosis by Coenzyme Q10 in Alloxan-induced Diabetic Rats. Journal Diabetes Complication. 26(6):476-82.