Published date: Dec 03 2017

Journal Title: KnE Life Sciences

Issue title: The Veterinary Medicine International Conference (VMIC)

Pages: 633-641

DOI: 10.18502/kls.v3i6.1192

Nur FaidahStudent of Veterinary Medicine Faculty, Surabaya 60115

Tatik HernawatiVeterinary Reproduction Department of Veterinary Medicine Faculty, Surabaya 60115

Mirni LamidVeterinary Animal Husbandry Department of Veterinary Medicine Faculty, Surabaya 60115

Ismudiono IsmudionoVeterinary Reproduction Department of Veterinary Medicine Faculty, Surabaya 60115

Tri Wahyu SuprayogiVeterinary Reproduction Department of Veterinary Medicine Faculty, Surabaya 60115

Sri MulyatiVeterinary Reproduction Department of Veterinary Medicine Faculty, Surabaya 60115

This research was determined membrane integrity and acrosome cap of Limousin bull post thawing after adding seawater extract with different concentrations in extender skim milk and egg yolk to increased frozen semen quality. This research used fresh samples of Limousin bull’s semen collected by using artificial vagina, then devided into 4 treatments and 6 replications. The experimental design that used was Complete Random Design. Analysis of the data using Analysis of Variant (ANOVA) one way then proceeds to the Duncan Multiple Range Test to determine significant differences between treatments.The first treatment P0 no seawater extract added as a control. The second treatment P1 was treated with 0.109 µL seawater extract, P2 was treated with 0.426 µL seawater extract, and P3 was treated with 1.09 µL seawater extract. The result showed that determine significant differences between treatments. The post thawing membrane integrity’s result was P0= 22.00 ± 4.28, P1= 22.66 ± 3.61, P2= 25.00 ± 2.75, and P3= 29.00 ± 1.67. The post thawing acrosome cap’s result was P0= 30.50 ± 1.37, P1= 31.50 ± 3.27, P2= 34.83 ± 2.31, and P3= 38.00 ± 1.41. The highest concentration added seawater extract to increased membrane integrity and acrosome cap spermatozoa in this research was 1.09 µL.

[1] Hardijanto, S. Susilowati, T. Hernawati, T. Sardjito dan T.W. Suprayogi. 2010. Inseminasi Buatan. Fakultas Kedokteran Hewan Universitas Airlangga. Airlangga University Press. Surabaya.
[2] Andrey Lyashenko. 2015. Effect of Different Thawing Procedures on the Quality and Fertility of the Bull Spermatozoa. National Academy Of Agrarian Sciences, Ukraines.
[3] Binod Kumar Dutta Borah, Bharat C.D., Ranjan Kumar B., and Kutubuddin Ahmed. 2015. Effect of Thawing Methods on Frozen Semen Quality of Yak (Poephagus grunniens L) bulls. Veterinary World, EISSN.
[4] Hafez, E. S. E. and B. Hafez. 2000. Reproduction in Farm Animals. Philadelphia: Lippicott Williams & Wilkins.
[5] Lenzi, A., L. Gandini., F. Lombardo., M. Picardo., V. Mareska., E. Panfili., F. Tramer., C. Boitani and F. Dondero. 2002. Polyunsaturated Fatty Acids Of Germ Cell Membranes, Glutathione and Glutathione- Dependent Enzyme Phgpx: From Basic To Clinic. Contraception. 65: 301 – 304.
[6] Guthrie H.D. and G.R. Welch. 2012. Effects of reactive oxygen species on sperm function.Theriogenology. Beltsville, Maryland.
[7] Hernawati. 2014. Peran Dan Mekanisme Osteopontin Seminal Plasma Pada Proses Pembekuan Semen Terhadap Kualitas Semen Beku Sapi perah Friesian holstein.Universitas Airlangga, Surabaya.
[8] Chatterjee, S. and Gagnon C. 2001. Production of Reactive Oxygen Spesies by Spermatozoa Undergoing Cooling, Freezing, and Thawing. Mol Reprod Dev. 59: 451-458.
[9] Sarwono, S. dan Y.P. Saragih. 2003. Membuat Aneka Tahu. Penebar Swadaya, Jakarta.
[10] Gums, J.G. 2004. Magnesium in Cardiovascular and Other Disorders. Am J Health-Syst Pharm. 61:1569-76.
[11] Lapointe, L, Ahmad I, Buhr. M.M, Sirard M,A. 1996. Modulation of Postthaw Motility, Survival, Calcium Uptake, and Fertility of Bovine Sperm by Magnesium and Manganase. Departement of Animal Poultry Science, University of Guelph. Canada.
[12] Waluyo, S.T. 2006. Pengaruh Penggunaan Prolin dalam Pengencer Susu Skim Pada Sperma Beku Terhadap Kualitas Sperma Domba Priangan. J.Anim. Prod. 8: 22 – 27.
[13] Hafez, E. S. E. and B. Hafez. 2000. Reproduction in Farm Animals. Philadelphia: Lippicott Williams & Wilkins.
[14] Rasul Z, Ahmad N, Anzar M. 2001. Changes in Motion Characteristics, Plasma Membrane Integrity and Acrosome Morphology During Cryopreservation of Buffalo Spermatozoa. J Androl. 22:278-283.
[15] Baqir, M., M.R. Fakhrildin, dan B.K. Kouty. 2009. Outcomes of Sperm Parameters, Hypo-Osmotic Swelling Test and Intra-Uterine Insemination For Varicocelic and Non-Varicocelic Infertile Patients. Journal Dohuk University.
[16] Eva Tvrda, Eva Tusimova, Anton Kovacik, Dusan Paal, Hana Greifova, Abzal Abdramanov, Norbert Lukac. 2016. Curcumin has Protective and Antioxidant Properties on Bull Spermatozoa Subjected to Induced Oxidative Stress. Kazakhstan.
[17] Malik Abdul, Yayan, M. Iwan Zakir, and M. Syarif Djaya. 2016. Effects of Addition of Juice Date Palm to the Extender on the Semen Qualities of Frozen Thawed in Bull Spermatozoa. Islamic University of Kalimantan, Indonesia.
[18] Murray, R.K., et al. 2000. Harper’s Biochemistry 25thed. Appleton and Lange. America. 534-626.