KnE Life Sciences
ISSN: 2413-0877
The latest conference proceedings on life sciences, medicine and pharmacology.
Antiviral Activity Effect of Silver Nanoparticles (Agnps) Solution Against the Growth of Infectious Bursal Disease Virus on Embryonated Chicken Eggs with Elisa Test
Published date: Dec 03 2017
Journal Title: KnE Life Sciences
Issue title: The Veterinary Medicine International Conference (VMIC)
Pages: 536-548
Authors:
Abstract:
Infectious bursal disease virus is one of the strategic infectious disease in Indonesia. Despite disinfection and vaccination technology has been doing, the cases still frequently occur and it needs another alternative technology to be developed to against IBD virus. This research try to answer the problem, it examines the effect of antiviral activity of silver nanoparticles (AgNPs) solution against the growth of infectious bursal disease virus in embryonated chicken eggs with ELISA Test. The research has two methods, the first method is conducted by mixing a solution of AgNPs and IBD Virus, two hours before inoculated (preventive method) and the second method is the virus inoculated first, 48 hours later the AgNPs solution injected (therapy method). Each method has several dosage of AgNPs solution respectively 0 ppm (positive control 20 ppm, 40 ppm, and 50 ppm). Virus samples taken from the choriallantoic membrane (CAM) and the embryo by crushed method. Results based on the value of OD (optical density) ELISA Test and Statistical Test ANOVA General Linier Models Univariate with Post-Hoc Duncan 5%, both methods have no significant difference (p>0.05), it means the solution of AgNPs has good preventative and therapeutic characteristic. The mean of OD values also showed dosage of 20ppm is most effective dosage in against the growth of the virus, the dosage has significant difference (p<0.05). The decreasing amount of virus in CAM and in embryos were not significantly different (p>0.05), in both CAM and embryo AgNPs solution has good antiviral properties.
Keywords : Silver Nanoparticles, Antiviral, Infectious Bursal Disesase, ELISA, Embryonated Chicken Eggs
References:
Abidin, Zainal. 2002. Meningkatkan Produktivitas Ayam Ras Pedaging. Agro Media Pustaka.
AP 9080.08. Depok.
Abdou, H.M. 1989. Dissoution, Bioavailability and Bioequivalence. Easton, Pennsylvania: Mack Printing.
Adelwahab, K.S. and Z.N. Said. 2013. Antiviral Replication Agents. ISBN 978-953-51-1055-2.
DOI: 10.5772/53707. Cairo: German Rosas-Acosta.
Akoso, B.T. 1993. Manual Kesehatan Unggas. Yokyakarta: Penerbit Kanisius. 107-109.
Amin, A.K. 2013. Sintesis, Studi Kestabilan Dan Karakterisasi Koloid Ag Nanopartikel Dengan Reduktor Hidrazin Sulfat Dalam Media Kitosan. Yogyakarta: Universitas Gajah Mada.
Apriandanu, D.O., S. Wahyuni dan S.H. Haryono.. 2013. Sintesis nanopartikel perak menggunakan metode poliol dengan agen stabilisator polivinil alcohol. Jurnal MIPA 36(1) : 157-168 (2013) : Unnes. Semarang.
Ariyanta, K.A., S. Wahyuni dan Priatmoko. 2013. Preparasi nanopartikel perak dengan metode reduksi dan aplikasinya sebagai anti-bakteri penyebab infeksi. Indo J Sci Unnes. Semarang Asraf, S. 2005. Studies On Infectious Bursal Disease Virus. Disertasi.The Ohio University. Ohio.
Bahri, S., E. Masbulan dan A. Kusumaningsih. 2005. Proses praproduksi sebagai faktor penting dalam menghasilkan produk ternak yang aman untuk manusia. J. Litbang Pertanian 24:27-35.
Benton, W.J., M.S. Cover, J.K. Rosenberger and R.S. Lake. 1967. Physicochemical Properties of Infectious Bursal Agent of Chickens. Avian Dis. 11:438-439.
Budinuryanto, D.C., M.H. Hadiana, R.L. Balia, Abubakar dan E. Widosari. 2000. Profil keamanan daging ayam local yang dipotong di pasar tradisional dalam kaitannya dengan penerapan system HACCP. Laporan hasil penelitian lembaga penelitian Universitas Padjajaran di ARMP II Badan Penelitian dan Pengembangan Pertanian. Bandung.
Butcher, G.D. and R.D. Milles. 2003. Infectious Bursa Disease (Gumboro) In Commercial Broilers.
Castro, Eduardo A. and Haghi A.K. (2012). Nanoscience and Advancing Computational Methods in Chemistry : Research Progress. Canada-Argentina. IGI Global. Pg 170.
Chwalibog, A., E. Sawosz, T. Niemiec and M. Grodzik, 2009. Nanotechnology in poultry production. Can silver nanoparticles promote health and growth of chicken?. Midterm Research Report.
Cunningham, C. H. (1973). A laboratory guide in virology. Burgers publishing Co. Minnesota.
7th Edn. P155-169.
Delgado, C., M. Rosegrant, Steinfeld, Ehmis and C. Curbois. 1999. Livestock to 2020 : The Next
Revolution. Washington – Rome – Nairubi : IFPRI – FAO – ILRI.
Ditjenak (Direktorat Jendral Peternakan). 2006. Statistic Peternakan Tahun 2005. Jakarta:
Ditjenak.
Elechiguerra, JL., Burt JL., Morones JR., Camacho-Bragado A., Gao X., Lara HH., and Yacaman M.J. 2005. Interaction of Silver Nanoparticles with HIV-1. Texas-Mexico. Journal of Nanobiotechnology-BioMed Central.
Ernawati, R., A.P. Rahardjo, N.W. Sianita, J. Rahmahani, F.A. Rantam dan Suwarno. 2013. Petunjuk Praktikum: Pemeriksaan Virologik dan Serologik. Surabaya: Laboratorium Virologi dan Imunologi Departemen Mikrobiologi Fakultas Kedokteran Hewan Universitas Airlangga.
Fitrianingsih, R. 2012. Synthesis of Silver Nanoparticles by Chemical Reduction at Various Fraction of MSA and Their Structure Characterization. Malang. Academic Journal. AIP Conference Proceedings;2014, Vol. 1589, p257
Fransisca, 2012. Pengembangan prototype vaksin TB tahap identifikasi isolate M. tuberculosis, penyiapan antigen, uji imunogenitas dan pembuatan isolate M. tuberculosis, “dorman” in vitro. Jakarta. LAK: Badan Penelitian dan Pengembangan Kesehatan Tahun 2012.
Gaikward, S. Ingie A. and Galdiero M. 2013. Antiviral Activity of Mycosynthesized Silver Nanoparticles Against Herpes Simplex Virus and Human Parainfluenza Virus Type 3. International Journal of Nanomedicine. 2013;8;4303-4314. Doi:10.2147/IJN.S50070.
Galdiero, S., Falanga A., Vitiello M., Cantisani M., Marra V. and Galdiero M.. 2011. Silver nanoparticles as potential antiviral agents. Molecules. 16, 8894-8918. Naples.
Hirai, K. and S. Shimakura. 1974. Structure Of Infectious Bursal Disease Virus. J. Virol.14: 957
– 964.
Lara, H.H., L. Ixtepan-Turrent, E.N. Garza-Trevino and C. Rodriguez-Padilla. 2010. PVP-coated silver nanoparticles block the transmission of cell-free and cell-associated HIV-1 in human cervical culture. Journal of Nanobiotechnologi. San Nicolas de los Garza.
Lara, H.H., E.N. Garza-Trevino, L. Ixtepan-Turrent and D.K. Singh. 2011. Silver nanoparticles are broad-spectrum bacterial an virucidal compounds. singhd@wssu.edu. San Nicolas de los Garza.
Li, W.R., Y. Zhang, X. Yang, N. Chen, Y. Sun, Y. Zhao, C. Fan, Q. Huang, Y. Zhu and J. Li. Appl Microbiol Biotechnol. 2010, 85 (4), 1115-1122. Shanghai: China
Lubick, N. Nanosilver toxicity: ions, nanoparticles--or both? Environ. Sci. Technol., 2008, 42 (23), p 8617
Lukert, P.D. and Y.M. Saif. 2003. Infectious Bursal Disease. In: Diseases Of Poultry 11ThEd.
Saif, Y.M., H.J. Barnes And J.R. Glisson (Eds.). Iowa State University Press. Ames. Pp.
161 – 170.
Machdum, Nurvidia. 2009. Gumboro pada Ayam. Jakarta: Invovet.
Mehrasa, M., S.R. Zaker, B. Larki, M. mehmandoust, H. Baghshahi, M.H. Sekhavati and S. Gavanji. 2014. Computational Prediction and Analysis of Ineraction of Silver Nitrate with Peptidoglycans Associated lipoprotein (Pal). Rangpur. J Sci Res Env Sci. 2014, 2(1), 8-13.
Moura, L., V. Vakharia, M. Li and H. Song. 2007. In Ovo Vaccine Against Infectious Bursal Disease. Maryland. Int. J. Poult. Sci. 6: 770 – 775
Natte, S. and Natalie D. 2011 In-Vitro-In-Vivo Correlation Definition and Regulatory Guidance.
Baltimore. International Journal of Generic Drugs.
Oldenburg. S.J. 2012. Silver Nanomaterials: Properties and Applications. nanoComposix, Inc.
San Diego.
Pal, J. and M.K. Deb. 2012. Microwave synthesis of polymercoated silver nanoparticles by glucose as reducing agent. Indian J Chem Sci A 51 : 821-824. Chhattisgarh.
Partadiredja, M., W. Rumawas dan I. Suharyanto. 1983. Penyakit Gumboro Di Indonesia Dan Akibatnya Bagi Peternak Ayam. Hemerazoa 71(1): 29 – 33. Bogor.
Pedersen, Gordon. 2014. Preventing Viral Infection with Silver Nanoparticles. Salt Lake City: A New Fighting Chance: Silver Solution: A Quantum Leap In Silver Technology: How molecular structuring safely destroys bacteria, viruses and yeast.
Rahardjo, A.P. dan Suwarno. 2005. Imunogenesitas Protein VP2 Virus Infectious Bursal Disease Isolat Lokal sebagai Dasar Pengembangan Vaksin Sub Unit. Surabaya: Media Kedokteran Hewan Vol. 21 No. 1.
Riady, M. 2005. Upaya pengembangan industry peternakan nasional bebas dari penyakit-penyait strategis. Pros. Seminar Nasional Teknologi Peternakan dan Veteriner. Bogor : 12-13 September 2005. Puslitbang Peternakan, hlm : 3-9.
Rogers, J.V., Parkinson C.V., Choi Y.W., Speshock J.L. and Hussain S.M.. 2008. A Pleminiary Assessment of Silver Nanoparticle Inhibition of Monkeypox Virus Plaque Formation. Nanoscale Research Letters. Columbus.
Rosenberger, J.K., Y.M. Saif and D.J. Jackwood. 2008. Infectious Bursal Disease. In: Dufour-Zavala, L, Swayne, DE, Glisson, J. RJ, Pearson, E, Reed, WM, Jackwood, MW and Woolcock, PR, editors. A laboratory manual for the isolation and identification of avian pathogens 5th ed, American Association of Avian Pathologists; p. 188-190. Newark.
Saputra, A H., A. Haryono, J.A. Laksmono dan M.H. Anshari. 2010. Preparasi koloid nanosilver dengan berbagai jenis reductor sebagai bahan antibakteri. Depok : Jurnal Sains Materi Indonesia.
Suwarno, F.A. Rantam, R. Ernawati., N.W. Sianita, A.P. Rahardjo dan J. Rahmahani. 2010. ELISA. Teori dan Protokol. Departemen Mikrobiologi Fakultas Kedokteran Hewan Universitas Airlangga. Surabaya.
Tabbu, C. R. 2000. Penyakit ayam dan penanggulangannya. Penyakit bacterial, mikal dan viral Volume I. Yogyakarta: Penerbit Kanisius.
Tangenjaya, B. dan A. Djajanegara. 2002. Peternakan Indonesia tahun 2020 : Suatu Visi Agriculture and Rural Development Strategy Studi. ADB-3843. Bogor.
Van den berg, T.P. 2000 Infectious bursal disease (Gumboro Disease). Belgium: Rev Sci Tech Off Int Epiz 2000, 19(2), 527-543. Bruxelles, Belgium-Ploufragan, France.
Vindevogel, H., M. Gouffaux, G. Meulemans, J.P. Duchatel and P. Halen. 1976. Infectious bursal disease : distribution and persistence of the virus in inoculated chickens. Study on the transmission of the disease. Bruxelles.
Wahyuwardani, S., D.R. Agungpriyono, L. Parede dan W. Manalu. 2011. Penyakit Gumboro: Etiologi, Epidemiologi, Patologi, Diagnosis dan Pengendaliannya. Wartazoa vol. 21 No.3. Bogor.
Xiang, D.X., Q. Chen, L. Pang and C.L. Zheng. 2013. 2011. Inhibitory effects of silver nanoparticles on H1N1 influenza A virus in vitro. J Virol Methods. 2011;178(1–2):137– 142.
Xiang, D.X., Y. Zheng, W. Duan, X. Li, J. Yin, S. Shigdar, M.L. O’Connor, M. Marappan, X. Zhao, Y. Miao, B. Xiang and C.L. Zheng. 2013. Inhibition of A/Human/Hubei/3/2005 (H3N2) influenza virus infection by silver nanoparticles in
