Published date: Dec 03 2017

Journal Title: KnE Life Sciences

Issue title: The Veterinary Medicine International Conference (VMIC)

Pages: 443-449

DOI: 10.18502/kls.v3i6.1153

Mas Rizky A.A Syamsunarnorizky@unpad.ac.idDepartment of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Padjadjaran, Jln. Raya Bandung-Sumedang KM.21, West Java, 45363

Mirasari PutriProgram Study Of Biotechnology, Post Graduate School Universitas Padjadjaran, Jl. Dipati Ukur no. 35, Bandung

Tatsuya IsoCentral Laboratory Universitas Padjadjaran, Jl. Raya Bandung Sumedang Km.21 Jatinangor Sumedang, West Java 45363

Rini WidyastutiDepartment of Biochemistry, Universitas Islam Bandung, JL Hariang Banga, No. 2, Taman Sari Bandung Wetan, 40116

Ramdan PanigoroDepartment of Medicine and Biological Science, Gunma University Graduate School of Medicine,3-39-22 Showa machi, Maebashi, Gunma 371-8511

Masahiko KurabayashiLaboratory of Animal Reproduction and Artificial Insemination, Department of Animal Production, Animal Husbandry Faculty Jl. Raya Bandung Sumedang Km.21 Jatinangor Sumedang, West Java 45363

Brown Adipose Tissue (BAT) is a nonshivering thermogenesis organ during cold exposure. Peroxisomal proliferator activated receptor alpha (PPARa) is the member of the nuclear hormone receptor superfamily and primarily expressed in BAT and liver. PPARa is coordinated with uncoupling protein 1 (UCP1) to regulate fatty acid metabolism in BAT. Fatty acid binding protein (FABP)-4 and-5 play role in adaptive response under fasting and cold exposure. The purpose of this study was to investigate the expression of PPARa in mice with FABP4/5 deficiency (DKO). Wildtype (WT) and DKO mice were exposed to cold for 2 hours under fed or 20 hours-fasted conditions. BAT was collected and further mRNA level of PPARa was examined using quantitative real-time PCR. As the result, PPARa gene expression in WT mice were increased 50% and 100% in fed and fasted condition respectively after cold exposure. There was no alteration in PPARa expression in  BAT of DKO mice. As conclusion, The functional FABP-4 and -5 are necessary to modulate PPARa gene expression in Brown Adipose Tissue under acute cold exposure  

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