Published date: Dec 03 2017

Journal Title: KnE Life Sciences

Issue title: The Veterinary Medicine International Conference (VMIC)

Pages: 395-403

DOI: 10.18502/kls.v3i6.1148

M. Gandul Atik Yulianigandulatik@yahoo.comDepartement of Basic Veterinary Science Veterinary Medicine Faculty, Universitas Airlangga. Kampus C UNAIR Jl..Mulyorejo, Surabaya

Didik HandijatnoDepartement of Microbiology Veterinary Medicine Faculty, Universitas Airlangga. Kampus C UNAIR Jl..Mulyorejo, Surabaya

Sri Pantja MadyawatiDepartement of Reproduction Veterinary Medicine Faculty, Universitas Airlangga. Kampus C UNAIR Jl..Mulyorejo, Surabaya

Aeromoniasis disease or known Ulcer Diseases caused by Aeromonas hydrophila which in fish or shrimp often occurs in some embankment or in the Aquarium can cause sign patches – patches of bleeding can even cause economic loss and death if not promptly treated with treatment that accompanied the good sanitation. This disease affects many farmed freshwater fish in East Java. Previous research has managed to do the characterization of antigenic protein originating from the OMP (outer membrane protein) Aeromonas hydrophilla. Because it is felt necessary to do DNA sequensing encoder protein in question.To achieve these goals then do some exploratory research laboratoric methods, namely: identification of the bacterium Aeromonas hydrophilla fish from suspect infection by PCR, gel electrophoresis and Sequencing of gene fragments of the agarosa OMP A. hydrophilla isolates of East Java. The results obtained from this research is the result of agarosa gel electrophoresis in DNA chromosome Aeromonas hydrophila bacteria cells is carried out at a temperature of annealing 54^oC position 400 bp and the results of the sequencing primer that is used produces a sequence of nucleotides is around 355 nucleotides.

[1]. Abbas, KA, Lichtman, AH, Pober,JS, 2000. Cellular and mollecular immunology 4th ed. WB Saunders Company A Harcourt Health Sciences Company Philadelphia London New York St Louis Sydney Toronto.
[2]. Adams J M. and Cory S., 1998. The Bcl-2 Protein Family: Arbiters of Cell Survival. . Science, 281:1322-1326
[3]. Adisukresno, S. 1994. Petunjuk Pelaksanan penyakit Ikan. Direktorat Jenderal Perikanan. Direktori Bina Sumber Hayati, Jakarta.
[4]. Anonimus. 2001. Determinasi Bakteri Patogenik Penyebab Penyakit Ikan. Fakultas Pertanian. Universitas Gadjah Mada. Yogyakarta.
[5]. Aoki, T. 1999. Motile Aeromonads (Aeromonas hydrophila). In : Fish Disease Disorders. Viral, Bacterial, and Fungal Infection. Cab. Internation. Vol.3.
[6]. Austin, B and Austin, D.A. 1999. Bacterial Fish Pathogens, Disease of farmed and Wild Fish 3rd . Springer Praxis. Goldming.
[7]. Afrianto, Eddy dan Evi Liviawaty. 1999. Pengendalian Hama dan Penyakit Ikan. Yogyakarta: Penerbit Kanisius.
[8]. Asha, A., DK. Nayak, KM. Shankar and CV. Mohan. 2004. Antigen expression in Biofilm cell of Aeromonas hydrophilla employed in oral vaccination of fish. Fish and Shellfish Immunology. 16:429-43.
[9]. Beesley, JE (eds), 1995. Immunocytochemistry. IRL Press New York
[10]. Chu, WH. and CP. Lu. 2008. In vivo fish models for visualizing Aeromonas hydrophilla invasion pathway using GFP as a biomarker. J. Aquaculture. 277:152-155
[11]. Dirjen Perikanan Budidaya. 2010. Statistik Perikanan Budidaya IndonesiA
[12]. Departemen Kelautan dan Perikanan.
[13].Dooley, J.S.G and Trust, T.J. 1988. Surface Protein Composition of Aeromonas hydrophila Strains Virulent for Fish : Identification of a Surface Array Protein. J. bacteriol. Vol.170. No. 2. p. 499-506
[14]. Goldsby, AR, Kindt, TJ, and Osborne, BA, 2000. Kuby Immunology. WH Feeman and Company New York.
[15]. Gulmez, N and Aslan, S, 1999. Histological and histometrical investigation on bursa of Fabricius and thymus of native geese.Tr J of Veterinary and Animal Sciences 23, 163-171.
[16]. Holm, JA. 1999. Disease Prevention and Control. Manual of Salmoning Farming. Blackwell Science. London.
[17]. Holt, J.G, Krieg, N.R, Sneath, P.H.A, Staley, J.T, William, S.T. 1994. Bergey’s Manual of Determinative Bacteriology. Nineth Edition. Williams & Wilkins. A. Waverly Company, USA. P. 190-191; 254-255.
[18]. Irianto, A. 2003. Patologi Ikan Teleostei. Gadjah Mada University Press. Yogyakarta.
[19]. Kordi K.dan M. Ghufron. 2004. Budi Daya Perairan. Bandung: PT Citra Aditya Bakti
[20]. Kibenge, FSB, Dillon, AS, and Russel, RG. 1988. Biochemistry and immunology of IBDV. Journal of General Virology. 69 : 1757-1775.
[21]. Madigan, M.T, Martinko, J.M, and Parker, J. 2000. Biology of Microorganism. 9th Edition. Prentice-Hall International Inc, USA (New Jersey). p. 991.
[22]. Nabib, R, dan Pasaribu, F. 1989. Patologi dan Penyakit Ikan. Departemen P dan K. Direktorat Jenderal Perguruan Tinggi. Pusat Antar Universitas Bioteknologi, IPB-Bogor. Hal. 120-129.
[23]. Minnaganti, VR., PJ. Patel, D. Iancu, PE. Schoq and BA. Cunha. 2000. Necrotizing Fasciitis caused by Aeromonas hydrophilla. J. Heart and Lung. Vol 29 No. 4.
[24]. Sitanggang, M. 2002. Mengatasi Penyakit dan Hama Pada Penyakit Ikan. Agro Media Pustakja, Jakarta. hal. 127-133.
[25]. Swann, L. 1991. Diagnosis and Treatment of Aeromonas hydrophila Infection of Fish. Animal Disease Diagnostic Laboratory. Purdue University, Illinois, USA.
[26]. Taslihan, Arief, dkk. 2006. Layanan Jasa Diagnosis Patogen: Mikrobiologi, Histopatologi dan Biologi Molekuler dan Parasitologi untuk Mendukung Program Diseminasi Budidaya Udang (Balai Besar Pengembangan Budidaia Air Payau). Jepara: Departemen Kelautan dan PerikanaN.
[26]. Vivekanandhan, G., K. Savithamani, AAM. Hatha and P Lakshmanaperumalsalny. 2002. Antibiotic Resistence of Aeromonas hydrophilla isolated from marketed fish and prawn of south India. International Journal of Food Microbiology. 76:165-168