Published date: Dec 03 2017

Journal Title: KnE Life Sciences

Issue title: The Veterinary Medicine International Conference (VMIC)

Pages: 316-326

DOI: 10.18502/kls.v3i6.1140

Indah Laili RahmawatiMahasiswa Fakultas Kedokteran Hewan, Universitas Airlangga

Fedik Abdul RantamDepartemen Mikrobiologi Veteriner Fakultas Kedokteran Hewan, Universitas Airlangga

Wiwik TyasningsihDepartemen Mikrobiologi Veteriner Fakultas Kedokteran Hewan, Universitas Airlangga

The aims of this research is to determine pathotype according to composition amino acid cleavage site of F protein, and epitope prediction of F protein from Surabaya isolate. Samples were cloacal swabs of 28 backyard chickens from five wet markets in Surabaya, 9 ND positive isolates from Laboratory of Virology and Immunology Department Microbiology Airlangga University, and also positive LaSota isolates (ATCC). Samples were isolated on the embryonated chicken egg and identified by HA test. The viruses were confirmed as NDV by hemagglutination inhibition assay and RT-PCR with target 687 bp. Prediction of epitope were using online software IEDB. The result of epitope B cell prediction from each isolate showing 11-12 epitope B cell candidates in protein F. Thus, it could be concluded that ND virus which isolated from Surabaya has a chance as immunogen candidate, which can be developed as vaccine candidate.

Key words: Newcastle disease, Cleavage site, Fusion protein, Surabaya

de Leeuw, O.S., G. Koch., L. Hartog., N. Ravenshorst and B.P. Peeters. 2005. Virulence of Newcastle Disease Virus is Determined by the Cleavage Site of the Fusion Protein and by both the Stem Region and Globular Head of the Haemagglutinin-Neuraminidase Protein. The Journal of General Virology. 86: 1759-1769.
Guerra, R.E.S., R.N. Gomez., D.O.G. Alonso and S.R. Mendoza. 2015. An Overview of Bioinformatics Tools for Epitope Prediction: Implications on Vaccine Development. Mexico. Journal of Biomedical Informatics 405–414.
Kencana, G. A. Y., I Made, K., dan I Gusti, N. K. M. 2012. Peneguhan Diagnosis Penyakit Newcastle Disease Lapang pada Ayam Buras di Bali Menggunakan Teknik RT-PCR. Fakultas Kedokteran Hewan Udayana. Denpasar. Bali.
Meulemans, G., T.P. Van der Berg., M. Decaesstacker and M. Boschmans. 2002. Evolution of Pigeons Newcastle Disease Virus Strains. Avian Pathology. 2002 Oct;31(5): 515-519.
Miller P.J., C.L. Afonso., J.E. Attrache., K.M. Dorsey., S.C. Courtney., Z. Guo and D.R. Kapczynski. 2013. Effects of Newcastle Disease Virus Vaccine Antibodies on the Shedding and Transmission of Challenge Viruses. Developmental and Comparative Immunology 41: 505–513.
OIE (Office International des Epizooties). 2012. Newcastle Disease. Manual of Diagnostic Tests and Vaccines for Terrestrial Animals. Chapter 2.3.14.
Rantam, F. A. 2008. Immunologi Molekuler. Fakultas Kedokteran Hewan Universitas Airlangga Surabaya.
Samal, S.K. 2012. Role of Fusion Protein in Newcastle Disease Virus Pathogenesis. Faculty of the Graduate School of the University of Maryland. University of Maryland.
Tizard, I.R. 1987. Pengantar Imunologi Veteriner.AUP
Samal, S.K. 2011. Newcastle Disease and Related Avian Paramyxoviruses, In Samal SK, editor. (ed), The biology of paramyxoviruses. Caister Academic Press, Norfolk, United Kingdom. 69–114.
Wibawan, I.W.T., D.S. Retno., C.S. Damayanti dan T.B. Tauffani. 2003. Diktat Imunologi. Bogor. FKH-IPB.
Wibowo, M. H., W. Asmara dan C. R. Tabbu. 2006. Isolasi dan Identifikasi Serologis virus Avian Influenza dari Sampel Unggas yang Diperoleh di Daerah Istimewa Yogyakarta dan Jawa Tengah. J Sain Vet, Vol.24 No.1: 77-83.
Yuliana, I.K.W., G.A.Y. Kencana dan I.N. Suartha. 2015. Seroprevalensi penyakit tetelo pada Peternakan Itik dan Pasar Galiran di Kabupaten Klungkung, Bali. Jurnal Veteriner, Vol.16 No.3: 383-388.