Published date: Dec 03 2017

Journal Title: KnE Life Sciences

Issue title: The Veterinary Medicine International Conference (VMIC)

Pages: 266-277

DOI: 10.18502/kls.v3i6.1135

Mesia Margi MahardikaStudent Faculty of Veterinary Medicine, Universitas Airlangga

Sri Agus SudjarwoDepartement of Basic Veterinary Medicine Faculty of Veterinary Medicine, Universitas Airlangga

Setiawan KoesdartoDepartment of Parasitology, Faculty of Veterinary Medicine, Universitas Airlangga

The aims of this research are to know the concentration, exposure time and interaction between concentration and exposure time of Ocimum sanctum Linn. leaves ethanol extract which cause the most mortality toward Fasciola gigantica. Also to know its value of LC₅₀ and LC₉₀. The research was completely randomized design. There were five treatments. Each treatment was done in four replications and used 10 Fasciola gigantica. The observation and recording of dead Fasciola gigantica were done at 0, 2, 4, 6, 8 and 10 hours. Fasciola gigantica were declared dead if there was no movement when disturbed by anatomy tweezers and when dipped in slightly warm water (50ºC). The obtained data was analyzed using ANOVA Factorial and continued with Duncan Multiple Range Test. The result was 10% concentration and exposure time for 10 hours caused the most mortality toward Fasciola gigantica. However, the interaction between concentration and exposure time resulted that 5% concentration for 8 hours already caused the most mortality of Fasciola gigantica. Probit analysis was used to calculate the LC₅₀ and LC₉₀. The results were LC₅₀ of Ocimum sanctum Linn. leaves ethanol extract was 7.9% at 4 hours, 3.7% at 6 hours, 1.8% at 8 hours and 0.8% at 10 hours and the LC₉₀ was 8.4% at 10 hours.

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