Published date: Dec 03 2017

Journal Title: KnE Life Sciences

Issue title: The Veterinary Medicine International Conference (VMIC)

Pages: 241-251

DOI: 10.18502/kls.v3i6.1133

Kurnijasanti R

Winarti D

Wahyuni R.S.

Puguh K

Setyabudi Setyabudi

Sukmanadi Sukmanadi

Sugihartuti R

Damayanti R

Hidayati N

Rahmawati K

Sudjarwo A.S.

This research aims to know the effect of ethanol extract of Indian acalypha (Acalipha indica L) to histopathology of coronary artery of  male rat (Ratus norvegicus) with high fat diet. Twenty male rats (Rattus Norvegicus) males aged 3-5 months with mean weight 200 grams were divided into 5 groups and 4 replications. Group 1 (negative control) was only given standard feed and distilled on ad libidum. Four groups (Positive control, Treatment 1, 2 and 3) were made hypercholesterolaemia by administering 1 mL peroral pork oil for 56 days. On day 14, the cholesterol levels were checked. After hypercholesterolaemia, from day 15 to day 56, each group was given ethanol extract of Indian acalypha  peroral with a dose of 200 g / kg BW (P1), 400 g / kg BW (P2) and 800 g / kgBW (P3), whereas the positive control groups were not given ethanol extract of Indian acalypha. On day 57, the dying rat was dissected for the removal of the cardiac organ and the making of coronary artery histopathological preparation. Data on histopathologic observation of coronary artery were analyzed by the Kruskal Wallis test, and if there was a significant difference between treatment groups (p <0.05), then it continued with the Mann-Whitney test. The results showed that in infiltration of fat cells and endothelial cells that experienced damage treatment P1, P2, and P3 which were given extracts of Indian acalypha experienced decrease compared to positive control treatment groups. The result of statistical test of fat cells and endothelial cells in the coronary artery that experienced damage showed significant differences between treatment groups (p <0.05), whereas in the statistical test of coronary artery foam cells showed no significant differences between treatment groups (P> 0 , 05). From the research, it can be concluded that ethanol extract of Indian acalypha (Acalipha indica L) can improve histopathology coronary artery of rat fed high fat diet.

Adlhani E. 2014. Penapisan Kandungan Fitokimia Pada Buah Labu Kuning (Cucurbita moschata). Jurnal Teknologi Dan Industri Vol.3 No.1.
Bahri A. 2004. Dislipidemia Sebagai Faktor Resiko Jantung Koroner. Fakultas Kedokteran Universitas Sumatera Utara.
Corwin, J.C. 2009. Sistem kardiovaskular. Dalam: Yudha EK, dkk editor bahasa Indonesia. Buku Saku Patofisiologi. Edisi revisi 3. Jakarta: Penerbit Buku Kedokteran EGC; 2009: 471-48.
Grace A. 2009. Efek neurotherapi ekstrak air akar acalipha indica linn. Dosis 15 dan 20 mg secara eksvivo pada persambungan saraf otot gastrocnemius katak Bufo Melanostictus schneider (Skripsi). Fakultas Kedokteran Universitas Indonesia.
Hardjana T, Kartika, R Dan Tutik, R. 2016. Potensi Buah Salak (Sallacca edulis, R.) Sebagai Suplemen Hipolipidemik Ditinjau Dari Gambaran Histopatologi Jantung dan Hepar Mencit yang Diberi Diet Rendah Lemak. Jurnal Sains Dasar. Vol.5. No.1. 82-93.
Hermanto S, Muawanah A. 2008. Profil dan Karakteristik Lemak Hewani (Ayam, Sapi dan Babi) Hasil Analisa FTIR dan GCMS. Vol. 1(3)
Hellen, W. 2005. Dislipidemia- terapi obat. Terj. Mod. Diana Lyrawati, 2008.
Iqbal, A. M., Noviarsyah, R., Budiarto, I.T., Hermadji, M. B. 2012. Potensi Bunga Keramunting (Melastoma malabothricum L.) Terhadap Kadar Kolesterol Total dan Trigliserida pada Tikus Putih Jantan Hiperlipidemia yang Diinduksi Profiltiourasil. Vol. 1. No. 2.

Merisa, I.W. 2011. Pengaruh Pemberian Seduhan Kelopak Kering Rosella Ungu (Hibiscus sabdariffa) Terhadap Kadar Kolesterol Total Serum Tikus Hiperkolesterolemia.
Rahimatul U., Surya, D., Dewita F M. 2016. Pengaruh Pemberian Campuran Jahe Mmerah, Bawang Putih, Cuka Anggur dan Madu Terhadap kadar Kolesterol Total dan Histopatologis Pembuluh darah Aorta Jantung Tikus Putih Jantan. Jurnal Farmasi Higea. Vol. 8. No.2.
Rajasekaran, R A, Nishad. 2013 Antihyperlipidemic Activity Of Acalypha indica Linn. On Atherogenic Diet Induced Hyperlipidemia.
Reinaldo M, Marie, K dan Tanudjaja G.N. 2014. Gambaran Histologi Aorta Tikus Wistar dengan Diet Lemak Babi Setelah Pemberian Ekstrak daun sirsak (Annona Muricata L.. Jurnal E biomedik.Vol.2.No.2.
Riesanti, Diajeng G, Masdiana C P, Herawati. 2016. Kadar HDL, Kadar LDL dan Gambaran Histopatologi Aorta Pada Hewan Model Tikus (Rattus Norvegicus) Hiperkolesterolemia Dengan Terapi Ekstrak Air Benalu Mangga (Dendrophthoe pentandra). (Artikel Ilmiah). Program Studi Kedokteran Hewan Universitas Brawijaya. Malang.
Sherwood, L. 2010. Human Phisiologi from Cell to System. Canada : Brooks/Cole Cengange Learning.
Soeharto I. 2004. Penyaki jantung koroner dan serangan jantung. Jakarta : PT. Gramedia Pustaka Utama. Halaman. 226-243.
Suryohandoyo. 2003. Hitomorfometrical Analysis Of Coronary atherosclerosis Lessions Formation in Rat (Rattus Norvegicus) medel. Journal of Dentistry Indonesia 2013. Vol 20. No.3. 73-77.
Suarsa, N. 2005. Jurnal Veterinary. Fakultas Kedokteran Hewan Universitas Udayana Bali.
Utami A P, Harjana T. 2016.Pengaruh Pemberian Kromium Nitrat dan Kromium Klorida pada Tikus Wistar yang Diinduksi dengan Streptozotocin-Nicotinamide terhadap gambaran histologi jantung. Jurnal Biologi.Vol.5.No.6.
Wemay AM, Fatimawali, Wehantouw F. 2013. “Uji Fitokimia dan Aktivitas Analgesik Ekstrak Etanol Tanaman Kucing-kucingan (Acalyphaludica Linn.) pada Tikus Putih Bekna Galur Wistar (Rartus Norvegicus L)”, Jurnal Ilmiah Farmasi.
Waji R.A dan A. Sugrani. 2009. Flavoroid (Quercetin), Makalah Kimia Organik Bahan Alam, Fakultas Matematika dan Ilmu pengetahuan Alam, Universitas Hasanudin, Makasar.
WHO. 2004. Atlas Of Heart Disease and Stroke. Dalam Asnani. 2011.Pengaruh Pemberian Buah Naga (Hylocereus Polyrhizus) Terhadap Profil Lipid Darah Tikus Putih (rattus Norvegicus ) galur wistar yang Mendapat Diet Tinggi Lemak. thesis. Universitas Airlangga. Surabaya.
Wurdianing, I, S.A Nugraheni, Rahfiludin, Z. 2014. Efek Daun Sirsak (Anona Muricata Linn) terhadap Profil Lipid Tikus Jantan (Rattus Norvegicus).Vol. 3. No. 1.