Published date: Dec 03 2017

Journal Title: KnE Life Sciences

Issue title: The Veterinary Medicine International Conference (VMIC)

Pages: 205-211

DOI: 10.18502/kls.v3i6.1129

Widjiati Widjiatiwidjiati1962@gmail.comDepartment of Anatomy, Faculty of Veterinary Medicine Airlangga University, Surabaya

Epy Muhammad LuqmanFaculty of Veterinary Medicine Brawijaya University, Malang Campus C Airlangga University, Mulyorejo Surabaya

Portia SumarsonoDepartment of Anatomy, Faculty of Veterinary Medicine Airlangga University, Surabaya

Vitrification is freezing method with low temperature (-196ºC) using high concentrations of cryoprotectants with a view to preventing the formation of ice crystals that can damage cells and decrease the viability of the embryo blastomeres. Embryos post warming which has low viability when transferred to a recipient will decrease the pregnancy rate. Intracellular cryoprotectants used in vitrification is ethylene glycol, propanediol, or DMSO. The third type of cryoprotectants has different capacities to protect the morula and blastocyst stage embryos. This study aims to decide the exact type of cryoprotectants in protecting the morula and blastocyst stage embryos when vitrification process. Research methods were divided into three groups of cryoprotectants that group treatment 1 (P1): Ethylene Glycol 30% + Sucrose 1 M + Insulin Transferrin Selenium 15 mL, group treatment 2 (P2): Propanediol 30% + Sucrose 1 M + Insulin Transferrin Selenium 15 mL, treatment Group 3 (P3): DMSO 30% + Sucrose 1 M + Insulin Transferrin Selenium 15 mL. The data obtained were analyzed by one-way ANOVA. Results of research that use Propanediol at the morula stage embryo vitrification is not significantly different from the Ethylene glycol but significantly different from DMSO. Then use Ethylene Glycol at the blastocyst stage embryo vitrification significantly different with Propanediol and DMSO and DMSO Propanediol but usage is no different. The conclusion of this study is Propanediol used as cryoprotectants in the vitrification process morula stage embryos, while ethylene glycol used as cryoprotectants the blastocyst stage embryo vitrification process.

Keywords: vitrification; ethylene glycol; propanediol; DMSO; morula; blastocyst

B. Jin, K. Mochida, A. Ogura, E. Hotta, Y. Kobayashi, K. Ito, G. Egawa, S. Seki, H. Honda, K. Edashige, M. Kasai. Equilibrium vitrification of mouse embryos. Biol. Reprod. 82 (2010) 444-450.
A. Arav, D. Shehu and M. Mattioli. Osmotic and cytotoxic study of vitrification of immature bovine oocytes. J Reprod 99 (1993) 353-358.
S. Kaidi, S. Bernard, P. Lambert, A. Massip, F. Dessy, and I. Donnay. Effect of Conventional Controlled-Rate Freezing and Vitrification on Morphology and Metabolism of Bovine Blastocysts Produced In Vitro. Biology of Reproduction. 65 (2001) 1127-1134.
Y.W. Jeong., M.S. Hossein, D.P. Bhandari, Y.W. Kim, J.H. Kim, S.W. Park, E. Lee, S.M. Park, Y.I. Jeong, J.Y. Lee, S. Kim and W.S. Hwang. Effect insulin transferrin selenium in defined and porcine follicular fluid supplemented IVM media on porcine IVF and SCNT embryo production. J. Anim. Reprod. Sci 106 (2008) 13-24.
X. Zhang, K.M. Trokoudes, C. Pavlides. Vitrification of biopsied embryos at cleavage, morula and blastocyst stage. Reproductive Bio Medecine. 19 (2009) 526-531.
T. Al-Azawi, S. Tavukcuoglu, A.A. Khaki, and S.A. Hasani. Cryopreservation of human oocytes, zygotes, embryos and blastocysts: A comparison study between slow freezing and ultra rapid (vitrification) methods. Middle East Fertility Society Journal 18 (2013) 223-232.
J. Zhang , J. Cui , X. Ling , X. Li , Y. Peng , X. Guo , B.C. Heng & G.Q. Tong. Vitrification of mouse embryos at 2-cell, 4-cell and 8-cell stages by Cryotop method. J. Assist. Reprod. Genet. 26 (2009) 621–628.