Published date: Dec 03 2017

Journal Title: KnE Life Sciences

Issue title: The Veterinary Medicine International Conference (VMIC)

Pages: 197-204

DOI: 10.18502/kls.v3i6.1128

Vilda Carlenia WardaniStudent of Faculty of Veterinary Medicine, Universitas Airlangga Kampus C UNAIR, Jalan Mulyorejo,Surabaya-60115

Sri Pantja Madyawatisripantja_madyawati@yahoo.comDepartement of Veterinary Reproduction, Faculty of Veterinary Medicine – Universitas Airlangga Kampus C UNAIR, Jalan Mulyorejo,Surabaya-60115

Poedji Hastutiek3Departement of Veterinary Parasitology, Faculty of Veterinary Medicine- Universitas Airlangga Kampus C UNAIR, Jalan Mulyorejo,Surabaya-60115

The research aims to find out profile of crude protein tyrosine kinase on plasma membrane of Merino sheep spermatozoa. The research used fresh semen sample collected using artificial vagina technique, next it was centrifuged to separate spermatozoa from seminal plasma. Then, spermatozoa (pellet) was isolated. After obtaining spermatozoa isolate, analysis on protein tyrosine kinase using  SDS-PAGE method was conducted. The method to analyze protein using SDS-PAGE to separate protein based on molecular weight. Analysis on molecular weight was conducted by comparing  result of isolate tapes with protein marker tapes. Running on  SDS-PAGE gel isolate protein plasma membrane of Merino sheep spermatozoa in the research resulted in 13 protein tapes with average molecular weights of 115.44 kDa; 95.78 kDa; 78.22 kDa; 69.02 kDa; 62.33 kDa; 53.72 kDa; 40.56 kDa; 30.74 kDa; 21.16 kDa; 13.67 kDa; 10.85 kDa; 9.49 and 8.07 kDa kDa. From this result, the second tape with the molecular weight of  95.78 kDa was believed to be protein tyrosine kinase tape however it needs to be confirmed further using Western Blot method.

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