Published date: Dec 03 2017

Journal Title: KnE Life Sciences

Issue title: The Veterinary Medicine International Conference (VMIC)

Pages: 189-196

DOI: 10.18502/kls.v3i6.1127

Tri Wahyu Suprayogytwsuprayogi@gmail.comDepartment of Veterinary Reproduction, Faculty of Veterinary Medicine, Airlangga University, Surabaya

Suherni SusilowatiDepartment of Veterinary Reproduction, Faculty of Veterinary Medicine, Airlangga University, Surabaya

Tatik HernawatiDepartment of Veterinary Reproduction, Faculty of Veterinary Medicine, Airlangga University, Surabaya

Nowdays, the storage of buck semen in cold temperature have not satisfied yet  because in  buck’s seminal plasma contains phospholipase enzyme which can coagulated egg yolk in diluents.The specific aim of this study was to investigate the benefits of  L-Arginin amino acid in skim diluents to quality  buck’s spermatozoa on cold temperature. This researchutilized four treatment groups, namely Controlled group (P0): skim milk diluent without L-Arginin + buck’s semen; P1: skim milk diluents + L-Arginin 0,002M/ml + buck” semen; P2: skim milk diluents + L-Arginin 0,004 M/ml + buck’s semen and P3: skim milk diluents + L-Arginin 0,006 M/ml + buck’s semen. Then the samples stored in cold temperature (5^oC). The result showed that sperm motility, viability and membrane integrity were significantly different (p<0,05) among the treatments.The conclusion of this study is adding of L-Arginin Amino Acid in skim milk diluents maintain motility, viability and membrane integrity  buck’s sperm.

Keywords: L-Arginin, buck, cold temperature, motility, viability and membrane integrity

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