Published date: Dec 03 2017

Journal Title: KnE Life Sciences

Issue title: The Veterinary Medicine International Conference (VMIC)

Pages: 69-76

DOI: 10.18502/kls.v3i6.1116

Theresia Indah B.Lecturer Faculty of Dentistry University of Airlangga

Bambang SumaryonoLecturer Faculty of Dentistry University of Airlangga

Ketut SuarditaLecturer Faculty of Dentistry University of Airlangga

Amelia Putri R.Dentist Education Students Faculty of Dentistry, University of Airlangga Surabaya

Catfish (Clarias batrachus) oil contain the highest amount of omega-3-PUFA among other freshwater fish. The omega-3-PUFA in fish oil produced eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). It is known that EPA and DHA in essentials fatty acid (EFA) could improve BMP-2 expression. Bone morphogenetic protein-2 (BMP-2) is bone stimulator which capable of inducing differentiation of mesenchymal cells into osteoblast, stimulating bone formation in wound healing process of dental extraction. Purpose: To prove the increasing expression of BMP-2 after catfish (Clarias batrachus) oil application. Methods: We have used the post test only design in this research. There have been 21 Rattus novergicus as research samples, and those were divided into 3 groups, group KK as control, group KP1 was given catfish (Clarias batrachus) oil in 5% concentration, and group KP2 was given catfish (Clarias batrachus) oil in 10% concentration. Catfish (Clarias batrachus) oil were applied into the socket of dental extraction. Rat was decapulated 7 days after fish oil application and the jaw in the treated regions and control group were cut for immunohistochemistry examination to observe BMP-2 expression. Data was analyzed using one-way ANOVA test.  Result: There is significant difference increased of BMP-2 expression between control and treatment group. In samples given with 10% concentration of catfish oil had the most significant increase of BMP-2 expression. Conclusion: Catfish (Clarias batrachus) oil in 10% concentration could increase the expression of BMP-2 post dental extraction.

Key words: Catfish oil, BMP-2, dental extraction.

1. Csiszar, A, Smith, KE, Koller, A, Kaley, G, Edwards, JG, & Ungvari, Z 2005, "Regulation of bone morphogenetic protein-2 expression in endothelial cells: Role of nuclear factor-κB activation by tumor necrosis factor-α, H2O2, and high intravascular pressure ', Circulation.
2. Csiszar, A, Lehoux, S, & Ungvari, Z 2009, "Hemodynamic forces, vascular oxidative stress, and regulation of BMP-2/4 expression ', Antioxidants & Redox Signaling, vol. 11, No. 7, pp. 1683-97.
3. Sularsih & Wahjuningsih, E 2015, 'Bone morphogenetic protein-2 of Rattus norvegicus using chitosan gel with different molecular weight on wound healing of dental extraction', Dent. J. (Maj. Ked. Teeth). Vol. 48, no. 2, pp. 59-64.
4. Chedoloh, R, Karrila, TT, and Pakdeechanuan, P 2011, 'Fatty acid composition of important aquatic animals in Southern Thailand', the International Food Research Journal, vol.18, pp. 783-790.
5. Das, UN 2002, "Nitric oxide as the mediator of the antiosteoporotic actions of estrogen, statins, and essential fatty acids', Nitric Oxide and Osteoporosis, Pp. 88-93
6. Kajarabille, N, Diaz-Castro, J, Hijano, S, Lopez-Frias, M, Lopez-Aliaga, I, & Ochoa, JJ, 2013, 'A new insight to bone turnover: Role of omega-3 polyunsaturated fatty acids 'The Scientific World Journal, p. 6.
7. Kamat, SG & Roy, R 2015, 'Evaluation of antioxidant potential of Clarias batrachus oil in alloxan Induced Diabetic Mice (Mus musculus)', Diabetes & Metabolism, vol. 6, no.6.
8. Larjava, N 2012, Oral Wound Healing: Cell Biology and Clinical Management, Canada: Wiley-Blackwell.
9. Kumar, V., Abbas, A.K., Aster, J.C. 2013. Robbins Basic Pathology 9thEd. Elsevier Saunders: Philadelpia. Pp. 48, 53-4.
10. Watkins, BA, Li, Y, & Seifert, MF 2001 'Nutraceutical fatty acids as biochemical and molecular modulators of skeletal biology', Journal of the American College of Nutrition, vol. 20, no. 5, pp. 410s-416S.
11. Viegas, MN, Dias, J, Cancela, ML, & Laize, V 2012. 'Polyunsaturated fatty acids regulate cell proliferation, extracellular matrix mineralization and gene expression in a cell line gilthead skeletal seabrem' J. Appl. Ichtyol, vol. 28, Pp. 427-32.