Published date: Jun 07 2022

Journal Title: KnE Life Sciences

Issue title: The First Asian PGPR Indonesian Chapter International e-Conference 2021

Pages: 469–478

DOI: 10.18502/kls.v7i3.11153

S.K. Sudirgasudirga@unud.ac.idFaculty of Mathematics and Natural Science, University of Udayana, Indonesia

N.M.S. ParwanayoniFaculty of Mathematics and Natural Science, University of Udayana, Indonesia

The fig (Ficus septica) is a widespread wild plant whose leaf extract contains bioactive compounds with antifungal properties, including alkaloid and phenolic compounds, both of which are antioxidants. Colletothricum spp. is a pathogenic fungus found in cultivated plants that can cause anthracnose, a plant disease. Papaya (Carica papaya) is one of the cultivated plants that frequently displays symptoms of anthracnose disease caused by Colletothricum spp. The focus of this research was to determine whether F. septica leaf extract could inhibit the growth of Colletothricum spp. on C. papaya. The antifungal activity of F. septica leaf extract against Colletothricum spp. was measured using the well diffusion method. The extract concentrations, which were determined by measuring the extract’s MIC value, were 0.5%, 1%, 2%, 3%, 4% and 5% (b/v), with methanol and synthetic fungicides serving as controls. According to the findings, the MIC value of the F. septica leaf extract on Colletothricum spp. growth was 0.4%, with an inhibition zone of 2.37 mm. Meanwhile, the inhibition zones formed by the F. septica leaf extract treatment were 2.5 mm, 3.4 mm, 7.6 mm, 9.7 mm, 12.7 mm, and 13.5 mm, respectively, and 0 mm for the negative control and 30 mm for the positive control. According to the analysis of variance results, all of the treatments produced results that were significantly different from those of the negative control, and the 4% extract was the most effective in vitro. We conclude that F. septica crude leaf extract contains bioactive compounds with antifungal properties and it can be used as a safe alternative to control Carica papaya anthracnose disease.

Keywords: anthracnose, Carica papaya, Colletothricum spp., Ficus septica.

[1] Semangun H. Pengantar ilmu penyakit tumbuhan. Yogyakarta: Gadjah Mada University Press; 2004.

[2] . Sa’id EG. Dampak negatif pestisida, sebuah catatan bagi kita semua. Journal of Agrotek. 1994;2(1):71-72.

[3] . Sudirga SK, Suprapta DN, Sudana IM, Wirya IG. Antifungal activity of leaf extracts of Ficus septica against Colletotrichum acutatum the cause of anthracnose disease on chili pepper. Journal of Biology Agriculture and Healthcare. 2014;4(28):47-52. [

4] . Pitt J, Hocking IA. Fungi and food spoiladge. London: Blackie Academic and Professional Press; 1997.

[5] . Ardiansyah L. Daun beluntas sebagai bahan anti bakteri dan antioksidan. Bertia Iptek; 2005-05-31. Available from: http://www.berita_iptek.com/cetak_beritahp?kat=berita&id =33

[6] . Dickman MW. The fungi. New York: Academic Press; 1993.

[7] . Alexopoulos C and Mims CW. Introductory mycology. 4th ed. . New York: John Willey & Sons, INC.; 1996.

[8] . Patel RV, Joshi KR, Solanky KU, Sabalpara AN. Colletotrichum gloeosporioides: A new leaf spot pathogen of turmeric in Gujarat. Ind J Phytophatology. 2005;58(1):125- 131

[9] . Sudirga SK, Darmayasa IBG, Wiryatno J. Antifungal activity of Ficus septica leaf extract against Colletotrichum gleosporioides cause anthracnose diseases on local fruit in Bali. J.Biological and Chemical Research. 2017;34(1):201-207.

[10] . Suprapta DN. Pestisida nabati potensi dan prospek pengembangan. Denpasar: Pelawa Sari; 2014.

[11] . Masangwa JIG, Aveling TAS, Kritzinger Q. Screening of plant extract for antifungal activities against Colletotrichum species of common vean (Phaseolus vulgaris L.) and cowpea (Vigna unguiculata (L.) Walp). Journal of Agricultural Science. 2012;5(2):1-10.

[12] . Gawade AE, Gaikwad NS, Bale SR. Selective inhibitory in vitro activity of Aegle marmelos (L.) extract of plant fungal pathogen Colletotrichum acutatum. Journal of Bioscience Discovery. 2014;5(1):55-59.

[13] . Sayeed MA, Hossain MS, Chowdhury MEA, Haque M. In vitro antimicrobial activity of methanolic extract of Moringa oleifera Lam. fruits. Journal of Pharmacognosy and Phytochemistry. 2012;1(4):94-98.

[14] . Doughari JH, Obidah JS. In vitro antifungal activity of stem bark extracts of Leptadenia lancifolia. International Journal of Integrative Biology. 2008;3(2):111-117.

[15] . Astiti NPA, Suprapta DN. Antifungal activity of teak (Tectona grandis L.f.) leat extract against Athrinium phaeospermum (Corda) M.B.Ellis, the cause of wood decay on Albizia falcataria (L.) Fosberg. Journal of International Society for Southeast Asia Agricultural Sciences (ISSAAS). 2012;18(1):62-69.