Published date: Jun 07 2022

Journal Title: KnE Life Sciences

Issue title: The First Asian PGPR Indonesian Chapter International e-Conference 2021

Pages: 444–457

DOI: 10.18502/kls.v7i3.11151

I Wayan Sudarmaswayan41@yahoo.comAssessment Institute for Agricultural Technology of Bali, Bypass Ngurah Rai, Indonesia

A.A.N. Badung S.DAssessment Institute for Agricultural Technology of Bali, Bypass Ngurah Rai, Indonesia

This research involved the fermentation of robusta coffee with termite enzymes in Subak Pura Pengulu, Bongancina village, Busungbiu–Buleleng between May and December 2019. The raw materials included red pickled coffee, termite microbes, water, pH meter molasses and plastic. 100 kg of the coffee sample was added in each wet processing treatment. Methods included sample preparation, field observations, coffee harvesting, sorting, and post-harvest processing. A completely randomized design with four treatments and five replications was used to create high-quality products with distinct flavors and aromas. Physical quality, taste, caffeine content and amino acids were reported. According to the findings, the yields of wet coffee beans and dry samples without skin (ose) were 51.73% and 19.36%, respectively. In addition, in the specialty categories, the three-day fermentation period produced the best taste, with a score of 83.50. Furthermore, the defect value was specified as 13.4, water content as 12.5, caffeine content as 3.40% w/w, and the acids, including oleic and α-linoleic, were 51.147 and 0.587 ppm, respectively. According to the economic estimations, the three-day fermented coffee would produce an optimal profit of IDR 8,090,000.

Keywords: robusta coffee, termite, fermentation

[1] Hartono. Produksi kopi nusantara ketiga terbesar di dunia. Kemenperin.go.id; 2013 Nov 3. Available from: http://www.kemenperin.go.id/artikel/6611

[2] Asosiasi Eksportir dan Industri Kopi Indonesia. Industri kopi Indonesia. AEKI; 2014. Available from: http://www.aeki-aice.org/page/industri-kopi/id

[3] Badan Pusat Statistik Bali. Provinsi Bali dalam angka. Denpasar : Badan Pusat Statistik Provinsi Bali 2019.

[4] Simanjuntak R. Artikel ilmu bahan makanann bahan penyegar. Semarang: Universitas Diponegoro 2011.

[5] Rahardjo P. Panduan budidaya dan pengolahan kopi arabika dan robusta. Jakarta (ID): Penebar Swadaya; 2012

[6] Brinchi L. Production of nanocrystalline cellulose from lignocellulosic biomass. Journal Carbohydrate Polimer. 2013;94:154-159.

[7] Diba F, Khotimah S, Febriyana U. Isolation and identification cellulolytic bacteria from the termite Coptotermes curvignatus holmgren and Macrotermes gilvus hagen from secondary forest in West Kalimantan Indonesia. Paper presented at: Procedings of the 9th Passific Rim Termite Research Group Conference 27-28 February 2012.

[8] Gupta P, Samant T, Sahu A. Isolation of cellulose-degrading termite bacteria and determination of their cellulolityc potential. International Journal of Microbiology. 2012;10(115):1-5.

[9] Poudyal H, Panchal SK, Diwan V, Brown L. Omega-3 fatty acids and metabolic syndrome: Effects and emerging mechanisms of action. Progress in Lipid Research. 2011;50:372-387.

[10] Lin CC. Approach of improving coffee industry in Taiwan promote quality of coffee bean by fermentation. The Journal of International Management Studies. 2010;5:154–159.

[11] Hadipernata M, Nugraha S. Identifikasi fisik, kimia dan mikrobiologi biji kopi luwak sebagai acuan teknologi proses kopi luwak artificial. Paper presented at: Prosiding Seminar Nasional Insentif Riset Sinas; 2012 Nov 29–30; Bandung, Indonesia.

[12] Nugroho D, Mawardi S, Yusianto, Arimersetiowati R. Karakterisasi mutu fisik dan citarasa biji kopi arabika varietas maragogip (Coffea arabica L. var. Maragogype Hort. ex. Froehner) dan seleksi pohon induk di Jawa Timur. Pelita Perkebunan. 2012;28:1–13.

[13] Budiman H. Prospek tinggi bertanam kopi pedoman meningkatkan kualitas perkebunan kopi. Yogyakarta: Pustaka Baru Press; 2012.

[14] Ana F, Evi R, Kumoro AC. Penurunan kadar kafein dan asam total pada biji kopi robusta menggunakan teknologi fermentasi anaerob fakultatif dengan mikroba nopkor MZ-15. Jurnal Teknologi Kimia dan Industri. 2013;2(3):70-7.

[15] Muchtadi TR, Ayustaningwarno F. Ilmu pengetahuan bahan pangan. Bandung: Alfabeta; 2010.

[16] Oktadina FD, Bambang DA, Hermanto MB. Pemanfaatan nanas (Anannas comosus L. merr) untuk penurunan kadar kafein dan perbaikan cita rasa kopi dalam pembuatan kopi bubuk. Jurnal Keteknikan Pertanian Tropis dan Biosistem. 2013;3(1):265-268.

[17] Speciality Coffee Association of America. Coffee term from the Speciality Coffee Association of America. Boston: The Event Specialty Coffee of The Year; 2013.

[18] Saputra B. Analisis pengaruh pH dan lama fermentasi terhadap kadar etanol hasil hidrolisis jerami padi [Skripsi]. Malang: Universitas Brawijaya 2015.

[19] Febriliyani YR. Pengaruh teknik penyeduhan dan ukuran partikel kopi bubuk terhadap atribut sensori seduhan kopi robusta dampit menggunakan metode RateAll-That-Apply (RATA) [Skripsi]. Malang: Universitas Brawijaya; 2016.

[20] Guntoro S. Proses memproduksi kopi luwak probiotik. Proposal paten. Denpasar: Balai Pengkajian Teknologi Pertanian (BPTP) Bali; 2010

[21] Somporn C, Kamtuo A, Theerakulpisur P, Siriamompun S. Effects of roasting degree on radical scavenging activity, phenolics and volatile compounds of Arabica coffee beans. International Journal of Food Science and Technology. 2011;46:2287–2296.

[22] Wang N. Physicochemical changes of coffee beans during roasting [Master of Science Thesis]. Ontario: University of Guelph; 2012.

[23] Arruda NP, Hovell AMC, Rezende CM, Freitas SP, Couri S, Bizzo HR. Arabica coffee discrimination between maturation stages and post-harvesting processing types using solid phase microextraction coupled to gas charomatography and principal components analysis. Quimica Nova. 2011;34(5):819-824.

[24] Taba J. Coffee taste analysis of an espresso coffee using nuclear magnetic spectroscopy [Bachelor Thesis]. Eindhoven, Holland: Central Ostrobothnia University of Applied Sciences; 2012.

[25] Correa E C, Jiménez-Ariza T, Díaz-Barcos V, Barreiro P, Diezma B, Oteros R, Acheverri C, Arranz F J, Ruiz-Altisent M. Advanced characterisation of a coffee fermenting tank by multi-distributed wireless sensors: Spatial interpolation and phase space graphs. Food and Bioprocess Technology. 2014;2:46–54.

[26] Vaseji N, Mojgani N, Amirinia C, Iranmanesh M. Comparison of butyric acid concentration in ordinary and probiotic yogurt samples in Iran. Iranian Journal of Microbilogy. 2012;2:87–93.

[27] Ni J, Tokuda G. Lignocellulose-degrading enzymes from termites and their symbiotic microbiota. Biotechnology Advances. 2013;(31):838–850.

[28] Santikunaporn M and Danphitak S. Hydrodeoxygenation of linoleic acid on ni-mo catalyst. Thammasat International Journal of Science and Technology. 2010: 15(5): 1-6

[29] Setor K K, Zaccardi F, Karppi J, Kurl S and LaukkanenIs J A. High Serum LDL/HDL Cholesterol Ratio an Emerging Risk Factor for Sudden Cardiac Death? Findings from the KIHD Study. Journal of Atherosclerosis and Thrombosis. 2016: 1-9

[30] Nuraeni I, Hidayat H. Manajemen usaha tani. Jakarta: Universitas Terbuka; 2011.