Published date: Jun 07 2022

Journal Title: KnE Life Sciences

Issue title: The First Asian PGPR Indonesian Chapter International e-Conference 2021

Pages: 99–109

DOI: 10.18502/kls.v7i3.11111

Darwis SulemanDepartement of Soil Science, Faculty of Agriculture, Halu Oleo University, Kendari

Asrul Sanisaniasrul2001@yahoo.comDepartement of Mathematics, Faculty of Mathematics and Natural Sciences, Halu Oleo University, Kendari

Suaib SuaibDepartement of Agrotechnology, Faculty of Agriculture, Halu Oleo University, Kendari

Sri AmbardiniDepartement of Biology, Faculty of Mathematics and Natural Sciences, Halu Oleo University, Kendari

Nur Arfa YantiDepartement of Biology, Faculty of Mathematics and Natural Sciences, Halu Oleo University, Kendari

Dirvamena BoerDepartement of Agrotechnology, Faculty of Agriculture, Halu Oleo University, Kendari

Dewi Nurhayati YusufDepartement of Soil Science, Faculty of Agriculture, Halu Oleo University, Kendari

Husna FaadDepartment of Forestry, Faculty of Forestry and Environmental Sciences, Halu Oleo University, Kendari

In crop production, phosphorus (P) is the second most important limiting nutrient. However, due to precipitation reactions with Al3+, Fe3+ in acidic soil, or Ca2+ in alkaline soil, its availability in soil is severely limited. Microbes have recently been proposed as a means of increasing the bioavailability of soil phosphate for plants. The goal of this research was to isolate and identify phosphate solubilizing molds (PSM) from various plant rhizospheres, including gadung (Dioscorea hispida Dennst), maize (Zea mays L.), bamboo (Dendrocalamus asper), pineapple (Ananas comosus L.), and banana (Ananas indica L.). PSM was isolated in vitro and then diluted using the dilution plate technique with Pikovskaya’s solid medium. Five colonies were confirmed as PSM, namely Talaromyces aculeatus, Metarhizium anisopliae, Fusarium proliferatum, Mucor hiemalis, and Aspergillus niger, out of fourteen colonies formed from those rhizospheres. In the PVK solid medium, these isolates were capable of solubilizing insoluble P with a solubility range of 2.05 to 3.03. Talaromyces aculeatus (125.6 mg L-1), Metarhizium anisopliae (80.76 mg L-1) and Fusarium proliferatum (41.59 mg L-1) were the best P solubilizers, followed by Mucor hiemalis (9.51 mg L-1), and Aspergillus niger (7.85 mg L-1), respectively. The bioinoculants Talaromyces aculeatus and Metarhizium anisopliae had the most potential.

Keywords: Dendrocalamus asper, Molds, Phosphate, Rhizosphere, Solubilizer

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