KnE Life Sciences

ISSN: 2413-0877

The latest conference proceedings on life sciences, medicine and pharmacology.

Halotolerant N-Fixing Bacteria Isolates for Increasing the Biochemical Activity, Total Bacteria Population, N-Uptake and Rice Seedling Growth

Published date: Jun 07 2022

Journal Title: KnE Life Sciences

Issue title: The First Asian PGPR Indonesian Chapter International e-Conference 2021

Pages: 1–12

DOI: 10.18502/kls.v7i3.11044

Authors:

Mieke Rochimi SetiawatiEmail: m.setiawati@unpad.ac.id
Affiliation: Soil Science and Land Resources Department, Universitas Padjadjaran
Biography:

Nida Uli Al-AzmiyaEmail: N/A
Affiliation: Soil Sience Postgraduate Program, Faculty of Agriculture, Universitas Padjadjaran,
Biography:

Fiqriah Hanum KhumairahEmail: N/A
Affiliation: Environmental Management Study Program, Samarinda State Agricultural Polytechnic East Kalimantan
Biography:

Tualar SimarmataEmail: N/A
Affiliation: Soil Science and Land Resources Department, Universitas Padjadjaran
Biography:

Mieke Rochimi Setiawati - m.setiawati@unpad.ac.id

Nida Uli Al-Azmiya

Fiqriah Hanum Khumairah

Tualar Simarmata

Abstract:

Rice farming is hampered by saline soil, which contains a large amount of soluble salt. The high salt content inhibits plant growth and causes nitrogen nutrient deficiency, as well as sodium and chlorine ion poisoning. Beneficial microbes that have adapted to saline ecosystems (halotolerant) can reduce salinity’s impact on rice growth. Microorganisms known as halotolerant bacteria can survive in high salt environments by maintaining an osmotic balance. The use of halotolerant N-fixing bacteria (HNB) as biofertilizers is an attempt to boost nitrogen nutrients and rice plant productivity in saline land. The goal of this study was to see how effective HNB isolates were at increasing rice plant growth. The experiment was conducted in the greenhouse of Universitas Padjadjaran’s Faculty of Agriculture, using a randomized block design with 16 treatments (control, single, and consortia of inoculant) and was repeated three times. Rice seedlings were inoculated with HNB isolates and grown in a Fahraeus saline medium. The HNB consortium application increased plant height by 9.03 cm, root-shoot ratio by 0.92, IAA content by 0.475 g/mL, nitrogen content by 2.94%, and the total number of HNB isolates (Azotobacter, Azospirillum, Bacillus, and Stenotrophomonas) by 8.10x107 CFU/mL.

Keywords: Biofertilizer, consortia inoculum, N-fixing bacteria, rice seedling

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