KnE Life Sciences
ISSN: 2413-0877
The latest conference proceedings on life sciences, medicine and pharmacology.
DEVELOPMENT OF LABEL-FREE BIOSENSOR FOR DETECTING STEROID HORMONE CONCENTRATION IN FISH
Published date: Feb 01 2015
Journal Title: KnE Life Sciences
Issue title: International Symposium on Aquatic Product Processing (ISAPPROSH) 2013
Pages: 212-223
Authors:
Abstract:
A novel label-free immunosensor for detecting steroid hormone was developed. The principle of the sensor system is based on differences in the electrochemical activity induced by an immunoreaction that depends on the levels of steroid hormone in the sample. A gold electrode functionalized with 3-mercaptopropionic acid (MPA) self- assembled monolayer was used to fabricate electrochemical immunosensor. In addition, single-wall carbon nanotube (SWCNT) was selected to expandthe dynamic range of the sensor. The sensor was immersed into a sample solution and measurements were determined by cyclic voltammetry. Each electrochemical measurement including sample immerse only took about 15min. In this study, we introduced an application of our sensor in detecting 17, 20β- dihydroxy-4-pregnen- 3-one (DHP). The immunosensor showed a specific response to DHP, and the oxidation peak current linearly decreased in the range of 7.8-500.0pg ml-1 (without SWCNT) and 15.6-50000.0pg ml-1 (with SWCNT). The sensor system was then applied to monitor DHP of goldfish (Carassiusauratus) and was compared with the levels of the same samples determined using ELISA as the convention method. Blood plasma of fish was collected every 3h after administering a DHP inducer. A good relationship (coefficient: 0.934) was observed between DHP levels determined by both methods.
Keyword: Biosensor, Steroid hormone, Immunoassay, Fish, Single-walled carbon nanotube
References:
Asahina K,Kambegawa A, Higashi T. 1995. Development of a micro titer plate enzyme-linked immunosorbent assay for 17, 20β- dihydroxy-4-pregnen-3-one, a teleostgonadal steroid. Fish Sci. 61: 491-494
Bromage N, Porter M, Randall C. 2001. The environmental reguluation of maturation in farmed finfish with special referece to the role of photoperiod and melatonin. Aquaculture 197: 63-68
Bulukin E, Mecucci V, Minunni M, Pretti C, Soldani G, Masdini M. 2007. An optical immunosensor for rapid vitellogenin detection in plasma from carp (Cyprinuscarpio).Talanta 72: 785-790
Carmona-Osalde C, Rodriguez-Serna M, Olvera-Novoa MA, Gutierrez-Yurrita PJ. 2004. Gonadal development, growth and survival of the crayfish Procambarusllamasi at three different water temperature. Aquaculture 232: 305-316
Currie LA. 1968. Limits for qualitative detection and quantitative determination: application to radiochemistry. Anal Chem. 40: 586-593
DuboisE.A,SlobS, Zandbergen M.A, Peute J, GoosH.J.Th. 2001. Gonadal steroids and the maturation of the species-specific gonadotropin-releasing hormone system in brain and pituitary of the male African catfish (Clariasgariepinus). Comp. Biochem. Physiol. B 129B: 381-387
Endo H, Igarashi M, Banba A, Ohnuki H, Ushio H, Hayashi T, Ren H, Yoshizaki G. 2011. Electrode-based immunologic assay system to monitor oocyte maturation-inducing hormone in fish. Int. J. Environ. Anal. Chem. 91: 174-184
Haussmann MF, Vleck CM, Farrar ES. 2007. A laboratory exercise to illustrate increased salivary cortisol in response to three stressful conditions using competitive ELISA. Adv. Physiol. Educ. 31: 110-115
Huang CH, Sedlak DL. 1998. Analysis of estrogenic hormones in municipal wastewater effluent and surface water using enzyme-linked immunosorbent assay and gas chromatography/tandem mass spectrometry. Environ. Toxicol. Chem. 20: 133-139
Idler DR, Fagerlund UHM, Ronald AP. 1960. Isolation of pregn-4-ene-17-alpha, 20beta-diol-3-one from the plasma of Pacific salmon (Oncorhynchusnerka).Biochem.Biophys. Res. Commun. 2: 133-137
Iijima S. 1991. Helical microtubules of graphitic carbon. Nature 354: 56-58
Jarboe HH, Romaire RP. 1995. Effects of density reduction and supplemental feeding on stunted crayfish Procambarusclarkia populations in earthen ponds. J. World Aquac. Soc. 26: 29-37
Kagawa H, Young G, Nagahama Y. 1983. Changes in plasma steroid hormone levels during gonadal maturation in female goldfish Carassiusauratus. Bull. Jpn. Soc. Sci. Fish 49:1783-1787
King VW, Berlimsky LD, Sullivan CV. 1995. Involvement of gonadal steroids in final oocyte maturation of white perch (Moroneamericana) and white bass (M. chrysops): in vivo and in vitro studies. Fish Physiol. Biochem. 14:489-500
Li ZL, Wang S, Lee NA. 2004. Development of a solid-phase extraction—enzyme-linked immunosorbent assay method for the determination of estrone in water. Anal.Chim.Acta. 503:171-177
Lous SW, Aida K, Hanyu I, Sakai K, Nomura M, Tanaka M, Tazaki S. 1984. Endocrine profiles in the females of a twice-annually spawning strain of rainbow trout. Aquaculture 43: 13-22
Mason DW, Williams AF. 1980. The kinetics of antibody binding to membrane antigens in solution and at the cell surface. Biochem. J. 187: 1-20
Matsuyama M. 1997.Maturation inducing hormone of fish. Radioisotopes 46: 263-264
Moriwaki T, Kobayashi M, Aida K, Hanyu I. 1991.Changes in plasma gonadotropin and steroid hormone levels during ovulation induced by HCG treatment in female goldfish. Bull. Jpn. Soc. Sci. Fish 57: 41-43
Nagahama Y, Yamashita M. 1989.Mechanisms of synthesis and action of 17, 20β- dihydroxy-4-pregnen-3-one, a teleost maturation-inducing substance. Fish Physiol. Biochem. 7: 193-200
Ohata K, Yamaguchi S, Yamaguchi A, Matsuyama M. 2002.Biosynthesis of steroids in ovarian follicles of red seabream, Pagrus major (Sparidae, Teleostei) during final oocyte maturation and the relative effectiveness of steroid metabolites for germinal vesicle breakdown in vitro.Comp. Biochem. Physiol. B 133B: 45-54
Qiu LP, Wang CC, Hu P, Wu ZS, Shen GL, Yu RQ. 2010. A label-free electrochemical immunoassay for IgG detection based on the electron transfer. Talanta 83: 42-47
Rivas GA, Rubianes MD, Rodriguez MC, Ferreyra NF, Luque GL, pedano ML, Miscoria SA, Parrado C. 2007. Caron nanotubes for electrochemical biosensing.Talanta 74: 291-307
Saito R, Shinohara H. 2004. Foundation and application of carbon nanotubes.Baifukan Co., Ltd., Tokyo (in Japanese)
Su X, Chew FT, Li SFY. 2000. Piezoelectric quartz crystal based label-free analysis for allergy disease. Biosens.Bioelectron. 15: 629-639
Suzuki R, Yamaguchi M. 1977. Effect of temperature on maturation of a cyprinid loach. Bull. Jpn. Soc. Sci. Fish 43: 367-373
Tamura T, Itazawa Y, Oguri M, Hanyu I. 1991. Fish physiology introduction.KouseishaKouseikaku Co., Ltd., Tokyo
Truscott B, Idler DR, So YP, Walsh JM. 1986. Maturational steroids and gonadotropin in upstream migratory sockeye salmon. Gen. Comp. Endocrinol. 62: 99-110
Ulman A. 1998. Thin films: Self-assembled monolayers of thiols (thin films). Academic Press, New York, pp 45-65
Vidal JC, Espuelas J, Garcia-Ruiz E, Castillo JR. 2004. Amperometric cholesterol biosensors based on the electropolymerization of pyrrole and the electrocatalytic effect of Prussian-Blue layers helped with self-assembled monolayers. Talanta 64: 655-664
Viswanathan S, Wu L, Huang M, Ho JA. 2006. Electrochemical immunosensor for cholera toxin using liposomes and poly (3,4-ethylenedioxythiophene)-coated carbon nanotubes. Anal. Chem. 78: 1115-1121
Yamauchi K, Kagawa H, Ban M, Kasahara Y. 1984. Changes in plasma estradiol-17β and 17, 20β- dihydroxy-4-pregnen-3-one levels during final oocyte maturation of the masu salmon, Oncorhynchusmasou. Nippon Suisan Gakkaishi 50: 2137
Zhang L, Yuan R, Huang X, Chai Y, Tang D, Cao S. 2005. A new label-free amperometricimmunosensor for rubella vaccine.Anal.Bioanal. Chem. 381: 1036-1040
